Review



kind ko k2gfp cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC kind ko k2gfp cells
    Demonstration of TFM with FluoroCubes and fluorescent beads as fiducial markers. (a) (i) and (ii) bright-field image of a murine kidney fibroblast with GFP-labeled kindlin-2 <t>(Kind</t> <t>Ko+K2GFP</t> cell line) and the corresponding fluorescent image; (b) displacement correlation between the bead and FluoroCube channel resulting from the stage shift in the x direction. The black dots represent the shifts measured by the conventional KLT method from the FluoroCube channel as a function of the corresponding shifts in the bead channel. The fitted curve has a slope of 0.9975 with R 2 = 0.9940; (c) (i) and (ii) displacement magnitudes measured in the bead and FluoroCube channels, respectively; (d) correlation of the displacements shown in (c) (i,ii); the micrometer-to-pixel factor is 0.103 μm/pix; (e) (i) traction forces calculated from the displacements in (c) (i) with BFTTC; (e) (ii) traction forces calculated from the displacements in (c) (ii); (e) (iii) traction forces calculated from dual-channel optical flow tracking results (see Figure S7 ). All scale bars represent 12.5 μm.
    Kind Ko K2gfp Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kind+ko+k2gfp+cells/pmc13130962-231-6-3?v=ATCC
    Average 99 stars, based on 1547 article reviews
    kind ko k2gfp cells - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "Traction Force Microscopy with DNA FluoroCubes"

    Article Title: Traction Force Microscopy with DNA FluoroCubes

    Journal: Langmuir

    doi: 10.1021/acs.langmuir.5c04971

    Demonstration of TFM with FluoroCubes and fluorescent beads as fiducial markers. (a) (i) and (ii) bright-field image of a murine kidney fibroblast with GFP-labeled kindlin-2 (Kind Ko+K2GFP cell line) and the corresponding fluorescent image; (b) displacement correlation between the bead and FluoroCube channel resulting from the stage shift in the x direction. The black dots represent the shifts measured by the conventional KLT method from the FluoroCube channel as a function of the corresponding shifts in the bead channel. The fitted curve has a slope of 0.9975 with R 2 = 0.9940; (c) (i) and (ii) displacement magnitudes measured in the bead and FluoroCube channels, respectively; (d) correlation of the displacements shown in (c) (i,ii); the micrometer-to-pixel factor is 0.103 μm/pix; (e) (i) traction forces calculated from the displacements in (c) (i) with BFTTC; (e) (ii) traction forces calculated from the displacements in (c) (ii); (e) (iii) traction forces calculated from dual-channel optical flow tracking results (see Figure S7 ). All scale bars represent 12.5 μm.
    Figure Legend Snippet: Demonstration of TFM with FluoroCubes and fluorescent beads as fiducial markers. (a) (i) and (ii) bright-field image of a murine kidney fibroblast with GFP-labeled kindlin-2 (Kind Ko+K2GFP cell line) and the corresponding fluorescent image; (b) displacement correlation between the bead and FluoroCube channel resulting from the stage shift in the x direction. The black dots represent the shifts measured by the conventional KLT method from the FluoroCube channel as a function of the corresponding shifts in the bead channel. The fitted curve has a slope of 0.9975 with R 2 = 0.9940; (c) (i) and (ii) displacement magnitudes measured in the bead and FluoroCube channels, respectively; (d) correlation of the displacements shown in (c) (i,ii); the micrometer-to-pixel factor is 0.103 μm/pix; (e) (i) traction forces calculated from the displacements in (c) (i) with BFTTC; (e) (ii) traction forces calculated from the displacements in (c) (ii); (e) (iii) traction forces calculated from dual-channel optical flow tracking results (see Figure S7 ). All scale bars represent 12.5 μm.

    Techniques Used: Labeling



    Similar Products

    kind ko k2gfp cells  (ATCC)
    99
    ATCC kind ko k2gfp cells
    Demonstration of TFM with FluoroCubes and fluorescent beads as fiducial markers. (a) (i) and (ii) bright-field image of a murine kidney fibroblast with GFP-labeled kindlin-2 <t>(Kind</t> <t>Ko+K2GFP</t> cell line) and the corresponding fluorescent image; (b) displacement correlation between the bead and FluoroCube channel resulting from the stage shift in the x direction. The black dots represent the shifts measured by the conventional KLT method from the FluoroCube channel as a function of the corresponding shifts in the bead channel. The fitted curve has a slope of 0.9975 with R 2 = 0.9940; (c) (i) and (ii) displacement magnitudes measured in the bead and FluoroCube channels, respectively; (d) correlation of the displacements shown in (c) (i,ii); the micrometer-to-pixel factor is 0.103 μm/pix; (e) (i) traction forces calculated from the displacements in (c) (i) with BFTTC; (e) (ii) traction forces calculated from the displacements in (c) (ii); (e) (iii) traction forces calculated from dual-channel optical flow tracking results (see Figure S7 ). All scale bars represent 12.5 μm.
    Kind Ko K2gfp Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kind+ko+k2gfp+cells/pmc13130962-231-6-3?v=ATCC
    Average 99 stars, based on 1 article reviews
    kind ko k2gfp cells - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Demonstration of TFM with FluoroCubes and fluorescent beads as fiducial markers. (a) (i) and (ii) bright-field image of a murine kidney fibroblast with GFP-labeled kindlin-2 (Kind Ko+K2GFP cell line) and the corresponding fluorescent image; (b) displacement correlation between the bead and FluoroCube channel resulting from the stage shift in the x direction. The black dots represent the shifts measured by the conventional KLT method from the FluoroCube channel as a function of the corresponding shifts in the bead channel. The fitted curve has a slope of 0.9975 with R 2 = 0.9940; (c) (i) and (ii) displacement magnitudes measured in the bead and FluoroCube channels, respectively; (d) correlation of the displacements shown in (c) (i,ii); the micrometer-to-pixel factor is 0.103 μm/pix; (e) (i) traction forces calculated from the displacements in (c) (i) with BFTTC; (e) (ii) traction forces calculated from the displacements in (c) (ii); (e) (iii) traction forces calculated from dual-channel optical flow tracking results (see Figure S7 ). All scale bars represent 12.5 μm.

    Journal: Langmuir

    Article Title: Traction Force Microscopy with DNA FluoroCubes

    doi: 10.1021/acs.langmuir.5c04971

    Figure Lengend Snippet: Demonstration of TFM with FluoroCubes and fluorescent beads as fiducial markers. (a) (i) and (ii) bright-field image of a murine kidney fibroblast with GFP-labeled kindlin-2 (Kind Ko+K2GFP cell line) and the corresponding fluorescent image; (b) displacement correlation between the bead and FluoroCube channel resulting from the stage shift in the x direction. The black dots represent the shifts measured by the conventional KLT method from the FluoroCube channel as a function of the corresponding shifts in the bead channel. The fitted curve has a slope of 0.9975 with R 2 = 0.9940; (c) (i) and (ii) displacement magnitudes measured in the bead and FluoroCube channels, respectively; (d) correlation of the displacements shown in (c) (i,ii); the micrometer-to-pixel factor is 0.103 μm/pix; (e) (i) traction forces calculated from the displacements in (c) (i) with BFTTC; (e) (ii) traction forces calculated from the displacements in (c) (ii); (e) (iii) traction forces calculated from dual-channel optical flow tracking results (see Figure S7 ). All scale bars represent 12.5 μm.

    Article Snippet: Human foreskin fibroblasts (ATCC; SCRC-1041) and Kind Ko+K2GFP cells (Kind Ko immortalized murine kidney fibroblast reconstituted with green fluorescent protein-tagged kindlin-2 expression plasmids, generous gift from Prof. Dr. Reinhard Fässler, PMID: 26821125) were cultured at 37 °C and in 5% CO 2 in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, D6546), supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, F2442), 4 mM l -glutamine (Sigma-Aldrich, G7513), and 20 μg/mL gentamicin (Sigma-Aldrich, G1397).

    Techniques: Labeling